Varian FTIR microscope

The Varian FTIR microscope combines a traditional infrared interferometric optical bench with a microscope for vibrational spectroscopy and imaging of samples. Reflection and transmission capabilities with Cassegrain microscope objectives. Attenuated total reflection (ATR) capabilities above 600 cm-1. Grazing angle reflection (65°-85°) capable of analyzing very thin coatings. Step scan kinetics for nanosecond time scale resolution. Motorized x-y stage for FTIR imaging.

Veeco/ Horiba Nano-Raman / AFM

The combination Bioscope II atomic force microscope and Labram HR Raman system facilitates scanning probe and optical imaging, and spectroscopy. Inverted and upright microscopes. Digital cameras for visible white light images. Multiple objectives: 10×, 20×, 50×, 60× oil immersion, 100×. Multiple laser wavelengths: 473 nm, 532 nm, 633 nm. Two gratings for high spectral resolution and/or fast acquisition of wide spectral ranges. Two Peltier cooled CCDs for low noise and high sensitivity, one with electron-multiplication capability. Rapid beam scanner: Facilitates sampling large areas. Reduces average power density for light-sensitive samples. Multiple AFM imaging modes (contact and tapping) in liquid or air. "Q-control" tapping mode AFM. Force curves. Subnanometer topographical resolution. AFM data analysis and manipulation, e.g. roughness and sectioning. Simultaneous Raman and AFM. Tip-enhanced Raman spectroscopy.

Pulse Interleaved fluorescence lifetime imaging microscope (PIE-FLIM)

Real time monitoring of fluorescence lifetimes with single molecule sensitivity and the capability of multiple configurations such as: Förster Resonance Transfer (FRET) Fluorescence Lifetime Imaging (FLIM) Time Correlated Single Photon Counting (TCSPC) Fluorescence Correlated Spectroscopy (FCS) Pulse-Interleaved Excitation (PIE) FRET/FLIM Two-Focus Fluorescence Correlated Spectroscopy (2-F-FCS).

Beckman Coulter Particle size and zeta potential analyser

The Delsa Nano C is a combined particle size and zeta potential analyser with the facility to titrate pH and record zeta potentials of both suspensions and solid phase samples. Measure particles in the size range from <1nm to ~7 µm in diameter. Zeta potential analysis. Sample temperature control. Auto titrator for determination of isoeletric point and zeta potential. Accurate measurement of Zeta potential in aqueous and non aqueous dispersions. Measurement of Zeta potential of solid surfaces. Multiple ‘cells’ to suit sample types. Further information is available here.

Veeco Bioscope

The Veeco Bioscope facilitates simultaneous Atomic Force Microscopy and fluorescence imaging. It can perform AFM and Fluorescence of `dry' and `wet' samples using a heated & environmentally controlled liquid perfusion chamber. It has a wide scan range: X-Y scan range > 150 μm Z range > 15 μm. The AFM has many modes: Contact mode, Tapping mode or equivalent, Phase Imaging, Lateral (or Friction) Force, Microscopy, Force Modulation, Force Volume Imaging with up to 256x256 pixels, Force Spectroscopy. It has the capability to capture data at a high resolution (512-512 pixel density) and with a high data capture rate (50 MHz). Spatial depth resolution is 0.1 nm in air and 0.2nm in water. Samples can be held on 60mm petri dishes, 25mmx75mm glass slides or 25mmx25mm coverslips.

Farfield Dual Polarisation Interferometer

The Farfield Analight 4D Dual Polarised Interferometer is an analytical technique used to understand the real-time structure and behaviour of a wide range of biomolecular structures, thin films and nanosurfaces through quantitative measurement at ultrahigh resolution. It has been successful across a range of biophysical and surface science applications. Adsorption and/or immobilisation of biomolecules (real time) and in an aqueous environment in real time. Binding of small molecules to immobilised biorecognition elements in real time. Angstrom resolution. Quantitative information on the thickness and density/coverage of the immobilised layers. Quantitative measurement of biomolecule dimensions, density (refractive index) and mass change (down to 0.1 pg/mm²). Capability to measure conformational change in biomolecules (down to 0.02nm) in real time. Temperature control & stabilisation (range 20 °C to 40 °C; +/- 0.002 °C). Wide dynamic range for refractive index (1.0 - 1.5). Flow through or static measurement. Parallel measurements. Further information is available here.